hct8 cells  (ATCC)

Journal: Frontiers in Oncology

Article Title: Efficacy of an XPO1 inhibitor in combination with irinotecan in a preclinical colorectal cancer model

doi: 10.3389/fonc.2026.1721685

Figure Lengend Snippet: In vitro evaluation of sequential dosing of SN38 and/or eltanexor in CRC cell lines (HCT8, HCT116, LS1034, and HCT15). (A) Dosing strategies for in vitro viability assay. Cells were incubated with SN38 [10 nM] for the first 24 hours. Cells were then washed with PBS and incubated with eltanexor [100 nM] for an additional 48 hours. (B) Cell viability % measured by CellTiter Glo 2.0 and (C) heatmaps of the 4 CRC cell lines treated sequentially with SN38 [0 - 30 nM] followed by eltanexor [0 -100 μM]. Bliss synergy score was analyzed using SynergyFinder.

Article Snippet: Human CRC cell lines HCT8 (RRID: CVCL_2478), HCT15 (RRID: CVCL_0292), HCT116 (RRID: CVCL_0291), and LS1034 (RRID: CVCL_1382) were purchased from American Type Culture Collection (ATCC) (Manassas, VA; ).

Techniques: In Vitro, Viability Assay, Incubation

Journal: Frontiers in Oncology

Article Title: Efficacy of an XPO1 inhibitor in combination with irinotecan in a preclinical colorectal cancer model

doi: 10.3389/fonc.2026.1721685

Figure Lengend Snippet: (A) A Western blot analysis of RAD51, p53, p-H2A.X and MSH2 in CRC cell lines HCT8, HCT116, LS1034, and HCT15 following sequential treatment of SN38 and/or eltanexor. (B) Densitometry analysis total cell protein. Cells were exposed to SN38 (10 nM) or vehicle for 6hr. (C) Cells were then washed with PBS and incubated with or without the presence of eltanexor (1 uM) for an additional 24 hr. Nuclear/Cytoplasmic. (D) Densitometry analysis of nuclear/cytoplasmic proteins.

Article Snippet: Human CRC cell lines HCT8 (RRID: CVCL_2478), HCT15 (RRID: CVCL_0292), HCT116 (RRID: CVCL_0291), and LS1034 (RRID: CVCL_1382) were purchased from American Type Culture Collection (ATCC) (Manassas, VA; ).

Techniques: Western Blot, Incubation

Journal: Frontiers in Oncology

Article Title: Efficacy of an XPO1 inhibitor in combination with irinotecan in a preclinical colorectal cancer model

doi: 10.3389/fonc.2026.1721685

Figure Lengend Snippet: Immunocytochemistry in HCT8 and HCT 116 cell lines. Cells were first treated with SN38 (10 nM) in time series (0 hr, 2 hr, 4 hr, and 6 hr). Cells were washed with PBS and then treated with eltanexor (1 uM) for 48 hours. Cells were fixed and then stained with p53 and p21 for cell cycle arrest or stained with P-H2A.X for double stained DNA breaks. (A) Schematic illustration of dosing strategy, (B) immunostaining in HCT8 cell line and (C) immunostaining in HCT116 cell line.

Article Snippet: Human CRC cell lines HCT8 (RRID: CVCL_2478), HCT15 (RRID: CVCL_0292), HCT116 (RRID: CVCL_0291), and LS1034 (RRID: CVCL_1382) were purchased from American Type Culture Collection (ATCC) (Manassas, VA; ).

Techniques: Immunocytochemistry, Staining, Immunostaining

Journal: Discover Oncology

Article Title: ATG7-induced autophagy inhibits ferroptosis and promotes the progression of colorectal adenocarcinoma

doi: 10.1007/s12672-025-03584-y

Figure Lengend Snippet: Autophagy-related ATG7 promotes the COAD proliferation and migration. a Expression and prognostic prediction of ATG7 in b tumor patients. c , d Detection of ATG7 expression in NIEC-8, HCT8, and SW620 cells. e , f Detection of overexpression efficiency of ATG7 in HCT8 and SW620 cells. g , h Detection of autophagy related proteins LC3II, Beclin1, and p62 expression after overexpression of ATG7 in HCT8 and SW620 cells. i The CCK8 experiment was used to detect the effect of overexpression of ATG7 on the proliferation of HCT8 and SW620. j The Transwell experiment was used to detect the effect of overexpression of ATG7 on the invasion of HCT8 and SW620. n = 3,** P <0.01, *** P <0.001

Article Snippet: The normal human intestinal epithelial cells, HIEC-6, along with the COAD cell lines HCT8 and SW620, and the HEK-293T cell line, are all sourced from ATCC (Manassas, VA).

Techniques: Migration, Expressing, Over Expression

Journal: Discover Oncology

Article Title: ATG7-induced autophagy inhibits ferroptosis and promotes the progression of colorectal adenocarcinoma

doi: 10.1007/s12672-025-03584-y

Figure Lengend Snippet: ATG7 involves in the COAD ferroptosis. a , b , c Detection of Fe2+, MDA, and GSH content after overexpression of ATG7 in HCT8 cells. d , e , f The content of Fe2+, MDA, and GSH in SW620 cellsafter overexpression of ATG7. g , h The ACSL4 and GPX4 protein expression in HCT8 and SW620 cells overexpressing ATG7. n = 3,** P <0.01, *** P <0.001

Article Snippet: The normal human intestinal epithelial cells, HIEC-6, along with the COAD cell lines HCT8 and SW620, and the HEK-293T cell line, are all sourced from ATCC (Manassas, VA).

Techniques: Over Expression, Expressing

Journal: Discover Oncology

Article Title: ATG7-induced autophagy inhibits ferroptosis and promotes the progression of colorectal adenocarcinoma

doi: 10.1007/s12672-025-03584-y

Figure Lengend Snippet: ATG7 controls the COAD ferroptosis by mediating the cell autophagy. a , b Analysis of the interaction between bATG7 and ferroptosis related proteins. c , d Detection of autophagy related protein expression after 3-MA treatment. e , f Analysis of Fe2+, MDA, and GSH content after 3-MA treatment. g , h Detection of ferroptosis related proteins ACSL4 and GPX4 expression after 3-MA treatment。 n = 3,* P <0.05 vs. Control, # P <0.05 vs. Vector + 3-MA

Article Snippet: The normal human intestinal epithelial cells, HIEC-6, along with the COAD cell lines HCT8 and SW620, and the HEK-293T cell line, are all sourced from ATCC (Manassas, VA).

Techniques: Expressing, Control, Plasmid Preparation

Journal: Discover Oncology

Article Title: ATG7-induced autophagy inhibits ferroptosis and promotes the progression of colorectal adenocarcinoma

doi: 10.1007/s12672-025-03584-y

Figure Lengend Snippet: Inhibiting autophagy can alleviate the inhibition of overexpressed ATG7-COAD cell ferroptosis treated with erastin. a The cell viability of CRC cells after treatment with erastin. b , c After treatment with erastin, the levels of Fe2+, MDA, and GSH in the cells were detected. d , e Analysis of ACSL4 and GPX4 protein expression in cells treated with erastin. f Analysis of HCT8 and SW620 cell proliferation after treatment with erastin. Analysis of HCT8 and SW620 cell invasion after treatment with erastin. n = 3,* P <0.05 vs. Control, # P <0.05 vs.Vector + erastin, & P <0.05 vs. ATG7 + srastin

Article Snippet: The normal human intestinal epithelial cells, HIEC-6, along with the COAD cell lines HCT8 and SW620, and the HEK-293T cell line, are all sourced from ATCC (Manassas, VA).

Techniques: Inhibition, Expressing, Control, Plasmid Preparation